Contact an Account ManagerSeparation of unsaturated fatty acids triacylglycerols and lipids by HPLC with a stable silver-loaded column
Summary
Type: HPLC
Application #: 100230
Catalog#: 720097.46 - MN HPLC Column, Nucleosil SA, 100A, 5um, 4.6x250mm
Detection: Fig. 1: light scattering detection,
Fig. 2: MS
Conditions: Fig. 1:
eluent A: 1,2-dichloroethane - dichloromethane (1:1, v/v), eluent B: acetone, eluent C: acetone - acetonitrile (9:1, v/v),
gradient started with 100% A, in 20min to 50% A, 50% B, then in 20min to 40% B, 60% C, finally in 20min to 100% C,
flow rate 1ml/min.
Fig. 2:
Conditions as above, gradient from A to 50% A - 50% B over 15min, then to 50% B - 50% C over a further 25min, and held at this for 5min.
Authors: Hoving, E.B.; Nikolova-Damyanova, B.; Christie, W.W.; Herslof, B.; Breckenridge, G.H.McG.
Source: J. Chromatogr. 565 (1991), 103–110
Details
Legend: J. Chromatogr. 565 (1991) 103 - 110; J.. Am. Oil Chem. Soc. 67 (1990) 503 - 507; J. Chromatogr. 469 (1989) 261 - 269; J. Chromatogr. 454 (1988) 273 - 284; HRC 10 (1987) 148 - 150